C3a, C4a, MMP-9, VIP, and MSH are important biomarkers involved various physiological and pathological processes, contributing to immune responses, inflammation, and neuroendocrine regulation (1).C3a and C4a are components of the complement system, playing crucial roles in immune responses.Elevated levels of C3a and C4a may indicate excessive immune activation, as seen in chronic inflammation (1).MMP-9 (Matrix Metalloproteinase-9) is an enzyme involved in tissue remodelling and repair (1). It is implicated in various pathological conditions, such as tissue degradation seen in arthritis and COPD.VIP (Vasoactive Intestinal Peptide) is a neuroregulatory hormone with anti-inflammatory properties. It regulates immune responses, gastrointestinal function, circadian rhythms, and inf lammatory responses, and is an important marker for those affected by mould exposure (1).MSH (Melanocyte-Stimulating Hormone) is part of the melanocortin system and plays a role in regulating inflammation, appetite, and skin pigmentation. Dysregulation of MSH is linked to conditions like obesity, skin disorders, sleep disorders and chronic pain (1).